【摘 要】
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该文主要研究了微生物催化法生产丙烯酰胺的工业化技术及相关的分离分析技术.包括分类鉴定高效的丙烯腈水合酶,选择合适的水合酶固定化工艺及固定化材料,开发固定化催化生产
论文部分内容阅读
该文主要研究了微生物催化法生产丙烯酰胺的工业化技术及相关的分离分析技术.包括分类鉴定高效的丙烯腈水合酶,选择合适的水合酶固定化工艺及固定化材料,开发固定化催化生产丙烯酰胺的发酵工艺,并对所得到的结果进行分离分析.论文对各种丙烯腈水合酶的活性进行了研究、对比、筛选,最后选用产酶活力高的86-163号菌株作为该研究的生产菌株,对该菌株的形态、特征、理化性质、活性以及所产酶的类型进行了较深入研究,确定该菌株是一种只生产丙烯腈水合酶的高选择性菌株.用不同方法对产酶菌株细胞进行了固定化研究,通过酶活回收率、细胞机械强度、催化丙烯腈水解反应的活性及循环使用次数对比确定了海藻酸钡包埋法为理想固定化方法.研究了介质pH值、温度、底物浓度、产物浓度等因素对含腈水合酶固定化细胞活性影响.最后采用固定化细胞催化法对丙烯腈水解生产丙烯酰胺进行了工业级发酵试验.结果表明,同化学法相比较,该工艺生产丙烯酰胺具有工艺简单,转化率和收率高,成本低,对环境基本无污染的优点.具有重大的经济价值.
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