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Genistein,genistin,daidzein,daidzin,glycitein and glycitin are major isoflavone compounds in Semen Sojae Praeparatum,which are believed to have benefical effects in preventive action against most chronic diseases.This study established an analytical method to determinethese six isoflavone components in rat plasma using liquidchromatography coupled to tandem mass spectrometry(LC-MS/MS).