京郊设施蔬菜黄瓜花叶病毒的检测

来源 :中国植物病理学会2014年学术年会 | 被引量 : 0次 | 上传用户：liwj

【摘要】

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【作者】

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陈柳冉策陈笑瑜邢冬梅胡学军尚巧霞

【机构】

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农业应用新技术北京市重点实验室/北京农学院植物科学技术学院,北京 102206 北京市植物保护站,

【出处】

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中国植物病理学会2014年学术年会

【发表日期】

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2014年3期

【关键词】

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ELISA RT-PCR CMV Detection

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　　蔬菜作物易于感染各种病毒,病毒病的发生已经成为蔬菜作物栽培生产的限制因素(尚巧霞,2009).黄瓜花叶病毒(cucumber mosaic virus,CMV)寄主范围非常广泛,是侵染我国十字花科、茄科、豆科及葫芦科等蔬菜上最主要的病毒之一(范卫红,2005).CMV传播途径极广,不仅可通过蚜虫非持久性传播,且极易通过汁液磨擦接种传播(王威麟,2010).目前,我国已从38科的120多种植物上分离到该种病毒(程朝玲,2013).ELLSA和RT-PCR具有检测速度快、灵敏度高、特异性强等优点,特别是核苷酸序列分析可将具有极其微小的两个或多个株系区分开,已成为鉴定CMV及其株系的可靠方法(李志勇,2005).

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京郊设施蔬菜黄瓜花叶病毒的检测

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