【摘 要】
：
The saffron (Crocus sp.) corms samples coming from Netherlands were screened by reverse transcription polymerase chain reaction (RT-PCR) assay using the degenerate primers (Sprimer and M4T) specific f
【机 构】
：
Inspection and Quarantine Technology Center, Xiamen Entry-Exit Inspection and Quarantine Bureau, Xia
【出 处】
：
中国植物病理学会2014年学术年会
论文部分内容阅读
The saffron (Crocus sp.) corms samples coming from Netherlands were screened by reverse transcription polymerase chain reaction (RT-PCR) assay using the degenerate primers (Sprimer and M4T) specific for potyviruses.A cDNA fragment with a predicted length of about 1.7 kb was amplified by RT-PCR, indicating the saffron corms were infected by a potyvirus.The sequence analysis revealed the highest sequence identity (65.3% ~98.6%) with the ornithogalum mosaic virus (OrMV) isolates.Phylogenetic analysis revealed the closest relationship of the isolate (designated 2677-NL) with five isolates of OrMV from Australia (JQ807995、 JQ807996、 NC_019409、 AF185964、 AF185965).Thus, the virus infecting saffron corms was identified as Ornithogalum mosaic virus.To our knowledge,this is the first report of natural infection of saffron by OrMV.
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