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Objective: To observe the effect of moxibustion on Du Meridian Gate of Yang-deficiency of infrared thermal imaging, to explore the correlation between the main and collateral channels and physical channels from the perspective of metabolic heat value, provide experimental evidence for the feasibility of Yang-deficiency and intervention for moxibustion.Methods: the constitution of traditional Chinese medicine after the judgment into yang deficiency, 11 volunteers, using infrared thermal imager to observe the process of Du moxibustion infrared thermal imaging changes compared before moxibustion, moxibustion, moxibustion, 10min 20min detection zone metabolism calorific value of infrared image, every 2min collected 1 times, a total of 11 times.Results: the infrared radiant track (IRRTM) observation: in the natural state, 2 volunteers IRRTM showed consistent with Du moxibustion gate line, after the continuous trajectory more regular, and it can make the remaining 9 cases of Yang deficiency induced by Du meridian line along the above 2/3 IRRTM, including 3 cases of Du meridian line throughout the region;temperature change detection: to Yang, the ridge, Yaoyangguan point moxibustion, moxibustion temperature in the 10min 20min temperature increased,the difference was statistically significant (P < 0.01 or P < 0.05), Shenzhu point in moxibustion acupoints at temperature higher than before, the moxibustion 20min is decreased slightly, but there were no significant difference.(P > 0.05).The relationship between the temperature change detection time: Moxibustion intervention, detection point temperature rise steadily to about 10min after moxibustion, reached a peak, and then tends to be steady, and continued to the end ofinoxibustion.Conclusion: the heat transfer characteristics and normal person is same with the Yang-deficiency constitution,Yang deficiency of Mingmen moxibustion can increase netabolic heat Du, can be used as the intervention method of Yang deficiency, the moxibustion time should be more than 10min, about 10-15min.