【摘 要】
：
目的 筛选人宫颈癌侧群细胞差异表达的microRNA及初步预测其特异基因.方法 取16例行宫颈鳞癌切除的手术标本,进行宫颈癌细胞原代培养,流式细胞仪分选侧群细胞(Side Population,SP),验证SP细胞的肿瘤干细胞特性,分别提取SP细胞和非SP细胞(Non Side Population,NSP)的总RNA,用microRNA芯片分别检测SP细胞和非SP细胞的microRNA表达谱并进
【机 构】
：
首都医科大学附属北京安贞医院妇产科
论文部分内容阅读
目的 筛选人宫颈癌侧群细胞差异表达的microRNA及初步预测其特异基因.方法 取16例行宫颈鳞癌切除的手术标本,进行宫颈癌细胞原代培养,流式细胞仪分选侧群细胞(Side Population,SP),验证SP细胞的肿瘤干细胞特性,分别提取SP细胞和非SP细胞(Non Side Population,NSP)的总RNA,用microRNA芯片分别检测SP细胞和非SP细胞的microRNA表达谱并进行分析,找出差异表达基因,用预测软件miRnada、Target Scan、Pictarmirbase对差异表达的microRNA进行靶基因预测.结果 在原代培养的16株宫颈癌细胞中均分选出SP细胞,比例约占1.67％±0.94％.microRNA芯片筛选出21条宫颈癌侧群细胞差异表达microRNA.其中,4条miRNA在SP细胞共同表达上调,17条miRNA在SP细胞共同表达下调.结论 人宫颈癌侧群细胞具有肿瘤干细胞特性,人宫颈癌侧群细胞表达一些特异的microRNA.
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