应用CRISPR/Cas9系统在鼠源、猪源和鲤鱼细胞系中对编码及非编码基因的特异性抑制的研究

来源 :中国细胞生物学学会2015年全国学术大会 | 被引量 : 0次 | 上传用户：mdyd888

【摘要】

：

CRISPR/Cas9是细菌和古细菌在长期演化过程中形成的一种适应性免疫防御,可用来对抗入侵的病毒及外源DNA.crRNA通过碱基配对与tracrRNA (trans-activating RNA)结合形成tracrRNA/crRNA复合物(gRNA),此复合物可引导Cas9核酸酶在与crRNA配对的序列靶位点造成DNA双链断裂.目前通过人工改造与设计RNA,理论上我们可以知道Cas9对大多数DN

【作者】

：

赵翊丞戴祯于泽欧阳红生滕春波

【机构】

：

东北林业大学生命科学学院,哈尔滨,150040 吉林大学动物科学学院,长春,130062

【出处】

：

中国细胞生物学学会2015年全国学术大会

【发表日期】

：

2015年4期

【关键词】

：

CRISPR/Cas9 猪鲤鱼 miRNA

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应用CRISPR/Cas9系统在鼠源、猪源和鲤鱼细胞系中对编码及非编码基因的特异性抑制的研究

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