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Correlative light and electron microscopy(CLEM) approach which guided the search for the structures of interest and allowed electron microscopy to zoom in on them bridged the gap between fluorescence light microscopy and electron microscopy.We set up a nonintegrated high-vacuum cryo-stage on epi-fluorescence microscope to develop CLEM imaging technology,which aimed to use fluorescence microscopy(FM) to identify areas of biological interests and apply electron microscopy(EM) to resolve ultra-structural details within those areas.