【摘 要】
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A novel method for the determination of aminoglycosides by surface-assisted laser desorption/ionization mass spectrometry (SALDI MS) with an aid of silver-coated gold nanoparticles (Au@AgNPs) has been
【机 构】
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Department of Chemistry, National Kaohsiung Normal University, Yanchao, Kaohsiung, Taiwan 824
【出 处】
：
第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会
论文部分内容阅读
A novel method for the determination of aminoglycosides by surface-assisted laser desorption/ionization mass spectrometry (SALDI MS) with an aid of silver-coated gold nanoparticles (Au@AgNPs) has been developed.The Au@AgNPs with surface capped by anionic citrate were used as concentrating probes as well as matrices in SALDI MS.Adsorption of aminoglycosides onto the nanoparticles was mainly through electrostatic attraction.The aminoglycoside-adsorbed nanoparticles were directly characterized by SALDI MS after a simple washing.Using Au@AgNPs to preconcentrate the aminoglycosides from 500 μL buffer solution, the limits of detection (LODs) at signal-to-noise ratio of 3 were 3, 25, 15, 30, and 38 nM for paromomycin, kanamycin A, neomycin, gentamicin and apramycin, respectively.This developed method was successfully applied to the determination of aminoglycosides in human plasma samples.The LODs of aminoglycosides in plasma samples were 9, 130, 81, and 180 nM for paromomycin, kanamycin A, neomycin, and gentamicin, respectively.Recoveries of aminoglycosides in plasma samples were about 80%.
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