Identification of the first diphenyl ether gene cluster for pestheic acid biosynthesis in plant endo

• 作 者: XinxinXu[1]LingLiu[1]FanZhang[1]WenzhaoWang[1]JinyangLi[1]LiangdongGuo[1]YongshengChe[2]GangLiu[1]
• 机 构: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 10
• 出 处: Asian Mycological Congress 2013 and The 13th International M
• 发表日期: 2013年期

　　The unique chloropupukeananes were isolated from an endophytic fungus Pestalotiopsis fici, and proposed as the Diels-Alder cycloadducts of co-isolated pestheic acid and iso-A82775C.The pestheic acid biosynthetic gene (pta) cluster was identified in the fungus through genome scanning.Sequence analysis revealed it encodes a non-reducing polyketide synthase, a number of modification enzymes and three regulators.Gene disruption and intermediate analysis demonstrated that the biosynthesis proceeded through formation of the polyketide backbone, cyclization of a polyketo acid to a benzophenone, chlorination, and formation of the diphenyl ether skeleton via oxidation and hydrolyzation.