10-Hydroxycamptothecin - induced apoptosis in silkworm BmN-SWU1 cells via the mitochondrial pathway

• 作 者: PANChun[1]HUYan-Fen[1]YIHua-Shan[1]SONGJuan[1]PANMin-Hui[2]LUCheng[2]
• 机 构: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China
• 出 处: 中国蚕学会第八届青年学术研讨会
• 发表日期: 2014年期

　　10-Hydroxycamptothecin (HCPT),a plant alkaloid separated from camptotheca acuminate,is known to be effective at inducing apoptosis of tumor cells; however,the mechanism is still unclear.In this study,we treated the ovarian cell line of the silkworm,BmN-SWU1,with different HCPT doses for durations ranging from zero to 72 h.The apoptosis morphology was evident after 72 h of incubation and included cell protuberance,concentrated cytoplasm and apoptotic bodies.We observed DNA fragmentation and cell apoptosis after HCPT treatment.The structural disruption of mitochondria,activation of the intracellular mitochondrial permeability transition pore,and release of cytochrome c during HCPT-induced apoptosis in dose and time-dependent manner indicates the involvement of mitochondria in BmN-SWU1 cells.Caspase-9 and -3 activities increased gradually with the duration of incubation time.In conclusion,HCPT induces apoptosis in BmN-SWU1 cells through a mitochondrial pathway,which may provide a reference point for future studies of the apoptotic signaling pathways in Lepidopteran insect cells.