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Objective: To investigate the efficacy of purging with rituximab in vivo combined with autologous peripheral blood stem cell transplantation (APBSCT) in patients with aggressive B-cell non-Hodgkin lymphoma.Methods: A total of 26 patients diagnosed with aggressive B-cell non-Hodgkin lymphoma in our hospital from January2005 to December 2012 were retrospectively analyzed.All patients received infusion of rituximab at a dose of 375 mg/m2for four times, including one before peripheral blood stem cell mobilization, one before stem cell collection, one before highdose therapy, and one on the day of leukocyte engraftment.