MicroRNA-23b promotes tolerogenic properties of dendritic cells in vitro through inhibiting Notch1/N

• 作 者: ZhengJ;
• 机 构: Department of Otolaryngology-Head and Neck Surgery, The First Affiliated Hospital to Guangzhou Medic
• 出 处: 广东省医学会第十六次耳鼻咽喉学学术会议
• 发表日期: 2012年期

　　BACKGROUND: MicroRNAs (miRNAs) are known to regulate the inflammatory response in various cell types.However, the ability of miRNAs to modulate dendritic cells (DCs) function for allergen immunotherapy is unclear.OBJECTIVE: To assess the role of miR-23b in the regulation of ovalbumin (OVA)-induced DC differentiation and function and to investigate the related molecular mechanisms.METHODS: Bone marrow-derived dendritic cells (BMDCs) were generated from murine bone marrow progenitor cells and subsequently stimulated with OVA to examine the profile of miRNA expression.After transfection with miR-23b reagents, DCs were evaluated for endocytic ability, surface marker expression, cytokine secretion and CD4+ T-cell differentiation.The possible roles of the Notch and NF-κ B signalling pathways were also evaluated.Human monocyte-derived dendritic cells (MDDCs) were similarly evaluated as well.