The effect of Lappaconitine and its synergistic effect with Docetaxol against Lung cancer In Vitro

• 作 者: Lianghe Sheng
• 机 构: Department of Oncology, The first affiliated hospital, Jinan University,Guangzhou, 510630, China
• 出 处: 广东省医学会第四届细胞治疗学学术会议
• 发表日期: 2016年8期
• 关键词: Lappaconitine cell cycle A549 Docetaxol Cyclin E1 VEGF-A

　　Method: The cytotoxic effect was determined by MTT assay.The cell apoptosis and cell cycle change was demonstrated by flow-cytometric analysis.The gene expression was detected by RT-PCR method.The morphological changes of A549 cell was investigated by atomic force microscope (AFM).Results (1)Lappaconitine at concentration of 0.48~3.6 mg/ml inhibited proliferation of A549 cells in vitro and was in a Dose dependent manner.0.8 μg/ml Docetaxol had significant proliferation inhibitory effect on A549.