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The prevalence of Clonorchis sinensis infection in freshwater fishes was surveyed in Heilongjiang Province,northeastern China,between August 2011 and September 2013.Thirteen species of freshwater fish(n =3221)and one species of shrimp(n =93)were collected from Songhua fiver,Nenjiang river and other lakes or ponds in 37 sites of 15 representative cities in Heilongjiang Province.They were individually examined by digestion technique,and the C.sinensis metacercariae were identified morphologically followed by confirmation using sequences of the second internal transcribed spacer of ribosomal DNA.Ten of the 13 examined species of freshwater fishes were infected with C.sinensis metacercariae,while all shrimps were negative.The overall prevalence of C.sinensis infection in 3221 examined freshwater fishes was 19.96%,with 42.57%(272/639)in Pseudorasbora parva,22.55%(83/368)in Hemicculter leuciclus,20.44%(121/592)in Carassius auratus,17.71%(68/384)in Saurogobio dabryi,10.85%(23/212)in Rhodeus ocellatus,10.54%(48/455)in Phoxinus lagowskii,8.20%(21/256)in Perccottus glehnii,6.25%(5/80)in Misgumus anguillicaudatus,4.55%(1/22)in Xenocypris davidi,and 1.49%(1/67)in Cyprinus carpio.The average infection intensity in P.parva was 103.3 encysted metacercariae per gram of fish meat in Zhaoyuan city.The average prevalence of C.sinensis infection in Songhua river,Nenjiang river and lakes or ponds were 31.96%(503/1574),11.30%(102/903)and 7.93%(59/744),respectively.The prevalence of C.sinensis infection in Zhaoyuan city(43.68%)was the highest among all sampling locations.These results revealed a high-prevalence of C.sinensis infection in freshwater fishes in Heilongjiang Province,northeastern China,posing significant public health concem.