Exploration of differential expressed genes involved in the development and progression of hepatocel

• 作 者: LIU Xiu-feng SHI Rui-hua GAO Heng-jun ZHU Hong QIN Shu-kui WANG Xue-hao
• 机 构: Department of Gastroenterology,the First Affiliated Hospital of Nanjing Medical University,Nanjing 2
• 出 处: 2007年生物芯片与疾病分子分型国际研讨会(International Symposium on Biochip Tec
• 发表日期: 2007年12期

　　objectives To analyze the differential wxpression genes(DEGs) among hepatocellular carcinoma(HCC),para-cancerous tissue(PCT) AND normal liver tissue(NLT) and explore the target genes related to the development and progression of HCC.Methods:The total RNAs of matched HCC,PCT and NLT of HCC patients were isolated using one step trizol method.Matched RNAs were qualified using 10g/l agarose gel electrophoresis and lab on chip.cRNAs were synthesized,flurescence labeled and purified after total RNAs were purified.The RNAs of HCC and NLT,HCC and PCT were hybridized with agilent oligomicroarray(21074 probes).The fluorescence indensity features were detected by agilent scanner and quantified by feature extraction software.The selected candidate genes were confirmed by SYBR Green Ⅰ strained real time PT-PCR.