Expression of p53,p16 and cyclooxygenase-2 in esophageal cancer detected by microarray

来源 :2007年生物芯片与疾病分子分型国际研讨会(International Symposium on Biochip Tec | 被引量 : 0次 | 上传用户:imlym
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  Objectives:Esophageal cancer development and progression is driven by the accumula-tion of genetic changes.In this study,we used tissue microarray to survey the expression of p53, p16and COX-2 in esophageal cancer progression and their clinical implication.Methods:We tested p53,p16and COX-2 proteins by immunohistochemistry and tissue microarray (Shanghai Outdo Biotech Co.,Ltd,SBC) in 86 specimens from different stagesof esophageal cancer and 40 specimens from adjacent non-cancer tissue.Results: The expression of p53and COX-2 was significantly higher in tumor tissue than that in non-tumor ones.As for the expression ofp16, no significant difference was found between tumor and adjacent tissue.An obvious relation wasobserved among p53, p16 and COX-2 expression that esophageal carcinogenesis was highly correlatedwith the positive expression of p53 or COX-2, however,no reciprocal relationship to neoplastic progression was recognized with p53, p16 and COX-2.
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