HP-PRRSV感染促进了猪体内HPS的增殖速度

来源 :2013国际猪繁殖与呼吸综合征学术研讨会 | 被引量 : 0次 | 上传用户：cklingdian

【摘要】

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【作者】

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于江吴家强张玉玉李俊杜以军彭军丛晓燕孙文博徐绍健时建立王金宝

【机构】

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山东师范大学生命科学学院,山东济南250014;山东省畜禽疫病防治与繁育重点实验室,山东济南250100

【出处】

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2013国际猪繁殖与呼吸综合征学术研讨会

【发表日期】

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2013年12期

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本研究的目的在于明确高致病性蓝耳病病毒(HP-PRRSV)在共感染中对副猪嗜血杆菌(HPS)的影响.根据HPS的一段保守序列(infB基因)设计引物,采用煮沸法从HPS培养物中提取DNA,进行PCR扩增.将鉴定正确的目的基因片段克隆入pMD 18-T载体中,转化大肠杆菌DH5α,经PCR及测序鉴定后得到阳性重组质粒,作为阳性标准品.通过敏感性、特异性、重复性试验,建立一种准确、快速的检测HPS的荧光定量PCR方法 .选用32头4周龄的猪,随机分成四组,一组用HP-PRRSV滴鼻5d后,腹腔注射HPS;二组单独用HP-PRRSV滴鼻;三组于第5天腹腔注射HPS;四组为对照组,腹腔注射生理盐水.

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HP-PRRSV感染促进了猪体内HPS的增殖速度

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