Study on the secretory function of Sertoli cells and testicular damage in diabetic rats

• 作 者: BaoxingLiu[1]XiupingZhang[1]MaoQin[1]AipingSong[2]TianweiCui[1]
• 机 构: Department of Andrology,China-Japan Friendship Hospital,Beijing 100029,China
• 出 处: 华夏医学论坛·泌尿生殖2015暨亚太性医学年会2015
• 发表日期: 2015年期

　　Objective: To investigate the changes of secretory function of Sertoli cells and testicular damage in diabetic rats.Methods: Diabetes was induced by intraperitoneal injection of streptozotocin(STZ,55 mg/kg/i.p.)in adult rats.Eight weeks later testicular tissue was harvested;serum INHB and testosterone(T)levels were measured by ELISA.The secretory proteins such as ABP,TF were measured by western blot.Testicular damage was detected by using hematoxylin and eosin(HE)staining and apoptosis was identified by terminal-deoxynucleotidyl-transferasemediated dUTP nick end labeling(TUNEL).Seminiferous tubular sperm formation was evaluated using Johnsens score.Results: In diabetic rats,ABP protein levels,serum INHB and T levels were found to be decreased gready.Meanwhile,TF protein levels decreased without significantly.Additionally,TUNEL-positive cells were increased and Johnsens score was decreased in diabetic testes.Conclusions: These results suggest that the secretory function of Sertoli cell in diabetic rats was damaged.The altered secretory function on Sertoli cell may be responsible for the disturbed spermatogenesis.