Purification, characterization and gene cloning of two marine bacterial catalases

• 作 者: WeiWang;XinhuaFu;MiSun;
• 机 构: Yellow Sea Fisheries Research Institute, Qingdao, Shandong Province 266071
• 出 处: 第十二届海洋药物学术年会
• 发表日期: 2015年期

　　The catalases from marine bacterium Bacillus sp.N2a (BNC) and Acinetobacter sp.YS0810(YS0810CAT) were purified and characterized.BNC from Bacillus sp.N2a isolated from Antarctic seawater has a molecular mass of about 230 kD and is composed of four identical subunits of 56 kD.The catalase showed optimal activity at 25 ℃ and at pH range of 6-11.The enzyme could be inhibited by azide, hydroxylamine and mercaptoethanol.The activation energy of BNC was 13 kJ/mol.The gene of psychrophilic catalase BNC was cloned by degenerate PCR and inverse PCR.