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Egg yolk phosphatidylcholine (EYPC) is being widely used in food and pharmacy industry nowadays because of its unique character.Common determination methods of phospholipids are based on the American Oil Chemists Society (AOCS) Official Method Ja7b-91 [1],which uses n-hexane/2-propanol/acetate buffer as the mobile phase and almost all the detection methods before were based on the following two solvent systems:hexane/2-propanol/water and acetonitrile/water (sometimes methanol added) mixtures.However,the buffer salt solution or water will create bad influence to the silica gel column both of its lifespan and the separation efficiency.In this study,we try to simplify the process of analyzing EYPC and avoid using water within the mobile phase.Using the mobile phase with no buffer will lead to incomplete separation and peak tailing.Changing mobile phase and using an evaporative light scattering detector can solve these problems.The final HPLC operating conditions is:pure methanol as a mobile phase,1.0 ml/min flow-rate,silica gel column (Inertsil SIL-100A,5urn,4.6*250mm),column temperature 30℃ and low temperature-evaporative light scattering detector was using.Under this condition,the standard curve linear relative coefficient is 0.998 and the recovery was in the range of 96.83%-101.58% with a relative standard deviation of 1.79% (n=6).