【摘 要】
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Background and Objective: Vascular smooth muscle cell(VSMC)migration occurs during vascular development in response to vascular injury and during atherogenesis[1].Conventional assays to study VSMC mig
【机 构】
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State Key Laboratory of Transducer Technology,Chinese Academy of Sciences,P.R.China
【出 处】
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第九届全国微全分析系统学术会议、第四届全国微纳尺度生物分离分析学术会议、2014国际微流控芯片与微纳尺度生物分离分析学术
论文部分内容阅读
Background and Objective: Vascular smooth muscle cell(VSMC)migration occurs during vascular development in response to vascular injury and during atherogenesis[1].Conventional assays to study VSMC migration cannot effectively reproduce VSMC microenvironments.Due to dimensional comparison with cells,microfluidics has been used to reconstruct local cellular environments,enabling migration studies of various cell types[2].However,the microfluidics based migration studies of VSMCs was not reported and thus in this study,the first microfluidic wound-healing assay enabling the quantification of VSMC migration was proposed.Method: Conventional soft lithography was used to fabricate Y-shape microfluidic channels and as shown in Figure(a),gravity was used to facilitate VSMC seeding and culture(a1),wound generation(a2),cell migration(a3)and on-chip staining(a4)in a tubing-free manner.Results and Conclusion: VSMCs were seeded in the microfluidic channel with monolayer confluence formed(b1),wound generated(b2),migration monitored(b3)and on-chip alpha actin immunostaining conducted(b4)(see Figure(b1)-(b4)).This microfluidic device may function as a new platform technology enabling the studies of VSMC migration.
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