【摘 要】
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A novel method,monoclonal surface display SELEX(MSD-SELEX),has been designed for simple,rapid,efficient,and cost-effective enrichment and identification of aptamers from a library of monoclonal DNA-di
【机 构】
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The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation,Innovation Center of Chemistry
【出 处】
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第九届全国微全分析系统学术会议、第四届全国微纳尺度生物分离分析学术会议、2014国际微流控芯片与微纳尺度生物分离分析学术
论文部分内容阅读
A novel method,monoclonal surface display SELEX(MSD-SELEX),has been designed for simple,rapid,efficient,and cost-effective enrichment and identification of aptamers from a library of monoclonal DNA-displaying beads produced via highly parallel single-molecule emulsion PCR.The approach was successfully applied for the identification of high-affinity aptamers that bind specifically to different types of targets,including cancer biomarker protein EpCAM and small toxin molecule aflatoxin B1.More importantly,the one-bead-one-sequence nature of the monoclonal beads allows rapid isolation and characterization of the binding affinity of individual sequences without sequence information.Compared with the conventional-cloning-sequencing-synthesis-screening workflow,the MSD-SELEX method based on highly parallel single-molecule emulsion PCR is simple,rapid,highly efficient,and cost-effective for aptamer enrichment and identification,which will greatly accelerate the aptamer discovery process to meet the growing demands for bioanalytical,biotechnological,and biomedical applications.
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