High sensitive genotyping of mutant K-ras in colorectal carcinomas with chip-based temperature gradi

• 作 者: HuidanZhang[1]RenHui[2]XiaonanWang[1]QianMa[1]ZheZhou[1]JinFang[1]
• 机 构: Department of Cell Biology, Key Laboratory of Cell Biology, Ministry of Public Health, and Key Labor
• 出 处: 第一届国际微纳尺度生物医学分离和分析技术学术会议暨第六届全国微全分析学术会议
• 发表日期: 2010年期

@@Background K-ras is a protooncogene that is frequently activated by point mutations in various cancers. In colorectal carcinomas（CRCs）, most of the K-ras mutations (90 %) are found at codons 12 and 13 in exon 1. The accurate detection is much valuable for early diagnosis and filtering out patients with CRCs benefiting from epidermal growth factor receptor (EGFR) - targeted therapy, which in turn increases the survival of CRC patients. However, the present gene-detecting techniques are of limited means for large-scale mutation detection due to low sensitivity, high cost, and time consuming procedures. In this work, a chip-based temperature gradient capillary electrophoresis (TGCE) method was applied to explore the possibility of high sensitive K-ras mutation detection and the feasibility of low-level mutant K-ras genotyping in CRCs.