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  5. Establishment of the high resolution melting analysis with unlabeled probes for genotyping CCL2-2518

Establishment of the high resolution melting analysis with unlabeled probes for genotyping CCL2-2518

来源 :GTAUF2014 & APSMHA2014第六届长城泌尿男科转化医学论坛暨第七届亚太男性健康与抗衰老研究学会年会 | 被引量 : 0次 | 上传用户：wangweiche
【摘 要】
：
Objective: To establish a high resolution melting analysis with unlabeled probes for genotyping CCL2-2518 T＞C.Methods: Two unlabeled probes were designed.One is complementary to wild type and another
【作 者】
：
Hongen Lei Zhezhu Gao Yongde Xu Zhongcheng Xin Yinglu Guo Ruili Guan 
【机 构】
：
Andrology Center, Peking University First Hospital, Peking University,Beijing 100034, China
【出 处】
：
GTAUF2014 & APSMHA2014第六届长城泌尿男科转化医学论坛暨第七届亚太男性健康与抗衰老研究学会年会
【发表日期】
：
2014年11期
【关键词】
：
high resolution melting analysis genotyping CCL2-2518 T>C clinical inspection 
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Objective: To establish a high resolution melting analysis with unlabeled probes for genotyping CCL2-2518 T＞C.Methods: Two unlabeled probes were designed.One is complementary to wild type and another is complementary to the mutant type.71 health people were performed by HRM with unlabeled probes for genotyping CCL2-2 518 T＞C, and TaqMan method was used to verify the results from above.
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