【摘 要】
：
In the HIV-1 replication cycle,the ESCRT(endosomal sorting complex required for transport)machinery facilitates viral budding and release in the late stages.In this process,the ESCRT proteins,ALIX and
【机 构】
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China State Key Laboratory of Medicinal Chemical Biology,College of Life Sciences,Nankai University,
【出 处】
：
中国生物化学与分子生物学会2016年全国学术会议
论文部分内容阅读
In the HIV-1 replication cycle,the ESCRT(endosomal sorting complex required for transport)machinery facilitates viral budding and release in the late stages.In this process,the ESCRT proteins,ALIX and TSG101,are recruited through interactions with HIV-1 Gag p6.ALG-2,also known as PDCD6,interacts with both ALIX and TSG101 and bridges ESCRT-Ⅲ and ESCRT-I.In this study,we show that ALG-2 negatively affects HIV-1 production at both the exogenous and endogenous level.Through a yeast-two hybrid screen,We identified HEBP2 as a new binding partner of ALG-2,and solved the crystal structure of the ALG-2/HEBP2 complex.The function of ALG-2/HEBP2 in HIV-1 replication was further explored.ALG-2 inhibits HIV-1 production by affecting Gag expression and distribution,and HEBP2 might facilitate this process by tethering ALG-2 in the cytoplasm.
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