【摘 要】
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Suppression of excessive inflammation can ameliorate blood brain barrier (BBB) injury, which shows therapeutic potential for clinical treatment of brain injury induced by stroke superimposed on system
【机 构】
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State Key Laboratory of Natural Medicines,Dept of Physiology,China Pharmaceutical University,Nanjing
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Suppression of excessive inflammation can ameliorate blood brain barrier (BBB) injury, which shows therapeutic potential for clinical treatment of brain injury induced by stroke superimposed on systemic inflammatory diseases.In this study, we investigated whether and how clematichinenoside (AR), an antiinflammatory triterpene saponin, protects brain injury from stroke superimposed on systemic inflammation.Lipopolysaccharide (LPS)was intraperitoneally injected immediately after middle cerebral artery occlusion (MCAO) in rats.Rat microvessel endothelial cells (rBMECs) were exposed to hypoxia/reoxygenation (H/R) coexisting with LPS.The results revealed that AR suppressed the excessive inflammation, restored BBB dysfunction, alleviated brain edema, decreased neutrophil infiltration, lessened neurological dysfunction, and decreased infarct rate.Further study demonstrated that the expression of nucleus nuclear factor kappa B (NFκB), inducible nitric oxide synthase (iNOS),intercellular adhesion molecule1 (ICAM1), tumor necrosis factoror (TNFo) and interlukin1 β (IL1 β) were suppressed by AR via zinc finger protein A20.Besides, AR increased in vitro BBB integrity through A20.In conclusion, AR alleviated cerebral inflammatory injury through A20NFκB signal pathway, offering an alternative medication for stroke associated with systemic inflammatory diseases.
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