【摘 要】
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We developed a set of novel tools to probe the dynamics of gene regulation and its coupling with chromosome conformation.We first developed a Co-Translational A
【机 构】
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JohnsHopkinsUniversity,USA
【出 处】
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8th IUPAP International Conference on Biological Physics(第八届
论文部分内容阅读
We developed a set of novel tools to probe the dynamics of gene regulation and its coupling with chromosome conformation.We first developed a Co-Translational Activation by Cleavage (CoTrAC) strategy to monitor the stochastic expression of a TF in its natural regulatory context in live E.coli cells at the single-molecule level.We further developed the CoTrAC strategy to enable the simultaneous monitoring of the real-time interplay of two TFs that mutually repress each other.Finally, we developed an in-vivo super resolution method to visualize the formation of DNA looping mediated by a transcription factor in live E.coli cells and correlated DNA looping with transcription regulation.The set of novel methods will open a wide door for quantitative investigations of the working of gene regulatory networks.
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