【摘 要】
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In present study M39 a potential E2 degrader was isolated from deep sea water (depth 208 meter) from South China Sea.The both microbiological and molecular characterization of isolated strain revealed
【机 构】
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Department of Biology, College of Science, Shantou University, Shantou, China
【出 处】
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第二十次全国环境微生物学学术研讨会
论文部分内容阅读
In present study M39 a potential E2 degrader was isolated from deep sea water (depth 208 meter) from South China Sea.The both microbiological and molecular characterization of isolated strain revealed that the isolated bacterial strain was bacillus in shape belongs to genus Pseudomonas,and class Gammaproteobacteria.The strain was gram negative in nature.The optimization of growth media and culture condition results of the M39 strain showed that it exhibited maximum growth and E2 degradation at 25℃C,pH 6 and 3% NaCl.It was seen that M39 bacterial cells metabolize E2 best (51%) when gown in the presence of sole carbon sources for 9 days.The degradation potential analysis was carried out by HPLC.During the E2 metabolism by M39 a new intermediate compound (COA-E1) was observed first time after 6 h of reaction.This compound was not accumulated during the reaction because there was no such compound found in later hrs of same reaction.The presence of this new intermediate compound was confirmed by GC-MS techniques.This study only showed the preliminary results about the finding out the new intermediate compound during E2 transformation to E1.
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