【摘 要】
：
Imprinting of insulin-like growth factor-2 gene(IGF2)is maintained by binding of CCCTC binding factor(CTCF)to the unmethylated imprinting control region(ICR)between IGF2 and H19 genes.We previously sh
【机 构】
：
Department of Biochemistry and Molecular Biology,West China School of Preclinical and Forensic Medic
【出 处】
：
中国生物化学与分子生物学会2016年全国学术会议
论文部分内容阅读
Imprinting of insulin-like growth factor-2 gene(IGF2)is maintained by binding of CCCTC binding factor(CTCF)to the unmethylated imprinting control region(ICR)between IGF2 and H19 genes.We previously showed that human high-density lipoprotein binding protein(HDLBP/vigilin)interacted with CTCF,and coexisted with CTCF at several CTCF binding sites of the ICR to regulate expression of IGF2.Here,we demonstrate that vigilin involves in maintaining the monoallelic expression of IGF2.We then identify that KH1-7 domain of vigilin is responsible for the vigilin-CTCF interaction,correspondingly,zinc finger domain of CTCF is involved in the interaction.Further,we discover that vigilin-CTCF interaction is RNA-dependent,and H19 long noncoding RNA(H19lncRNA)is one of the RNAs.Moreover,we investigate that H19 lncRNA also plays a important role in monoallelic expression of IGF2.Thus,H19 lncRNA may mediated interaction of vigilin and CTCF to participate in the maintenance of monoallelic expression at IGF2 gene locus.
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