【摘 要】
：
Radiotherapy is a major treatment measure for lung cancer therapy.However,acquired radioresistance of tumor cells during treatment always leads to therapy failure and tumor relapse,and the mechanisms
【机 构】
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Key Laboratory of Tumor Molecular Biology,Department of Biochemistry and Molecular Biology,Binzhou M
【出 处】
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中国生物化学与分子生物学会2016年全国学术会议
论文部分内容阅读
Radiotherapy is a major treatment measure for lung cancer therapy.However,acquired radioresistance of tumor cells during treatment always leads to therapy failure and tumor relapse,and the mechanisms of resistance to radiation are still unclear.MicroRNAs(miRNAs)modulate key cellular pathways that mediate response to radiation.To study the roles of miR-320a in the radioresistance of lung adenocarcinoma,we first found that miR-320a levels were much lower in the radioresistant lung adenocarcinoma cell lines(A549/34R)compared with those in control A549 cells.MiR-320a treatment effectively suppressed A549/34R cell proliferation and induced more apoptotic A549/34R cells in miR-320a-treated cells compared with control treatment.Second,we found that miR-320a treatment enhanced the sensitivity of radioresistant A549/34R cells to radiation compared with control treatment.MiR-320a,as a novel miRNA,regulated signal transducer and activator of transcription 3(STAT3)signals,such as Bcl-2,Bax,Caspase 3,etc.Third,both the results of siRNA inhibiting STAT3 and IL-6 enhanced STAT3 levels proved that the roles of STAT3 signals regulated by miR-320a in affecting the growth of radioresistant A549/34R cells.Last,our results further showed that miR-320a effectively suppressed A549/34R cell growth in xenografts of mice compared with scrambled control treatment.Moreover,we found that miR-320a effectively inhibited A549/34R cell migration in vitro and in vivo.Altogether,our findings demonstrate that miR-320a,as novel miRNA regulating STAT3 signals,not only suppresses radioresistant A549/34R cell growth and migration,but enhances the sensitivity of A549/34R cell to radiation in vitro and in vivo,which providing novel therapeutic targets for the radioresistant lung adenocarcinoma cells.
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