【摘 要】
：
克隆鱼腥草糖基转移酶UGT75C1基因并进行原核表达分析.根据已经获得的鱼腥草UGT75C1转录本序列设计1对引物,采用RT-PCR方法获得UGT75C1基因cDNA序列并对UGT75C1蛋白进行
【机 构】
：
民族药用植物资源研究与利用湖南省重点实验室湘西药用植物与民族植物学湖南省高校重点实验室怀化学院生命科学系,湖南怀化418008
论文部分内容阅读
克隆鱼腥草糖基转移酶UGT75C1基因并进行原核表达分析.根据已经获得的鱼腥草UGT75C1转录本序列设计1对引物,采用RT-PCR方法获得UGT75C1基因cDNA序列并对UGT75C1蛋白进行理化性质分析,并预测了该蛋白功能；利用实时荧光定量PCR方法检测了UGT75C1基因在鱼腥草的地下茎、地上茎、叶、花中的表达情况,将克隆得到的UGT75C1基因完整开放阅读框连接到原核表达载体pGEX4T-1上,转化大肠杆菌E.coli.BL21(DE3),通过IPTG诱导表达,SDS-PAGE检测表达产物.克隆获得的UGT75C1基因长为1 787bp,开放阅读框1 461bp,编码486个氨基酸.
其他文献
Despite small interfering RNA (siRNA) has garnered much interest as a potential drug for various diseases,the clinical application of siRNA still faces consider
2008年以来,依托以昆山小核酸研究所为核心,昆山构建小核酸产业技术创新平台,聚集专业服务和要素资源,奠定昆山小核酸产业发展的基础.面临全球小核酸技术产业化的关键节点,在
The application of nanotechnology to engineer nanovectors for siRNA delivery is widely expected to bring breakthrough in nanomedicine and create entirely novel
The most remarkable findings in the past two decades in biology include how the mammalian genome is largely transcribed and how versatile functions RNA molecule
Chromatin remodeling is an essential event for HIV-1 transcription.The HIV-1 transactivator,Tat,plays a major role in assembling a number of required transcript
The CRISPR RNA-guided Cas9 nuclease gene-targeting system has been successfully used for genome editing in a variety of organisms.Here,we report the use of
The application of nanotechnology for drug delivery is widely expected to bring promise and create novel therapeutics.[1] Particularly exciting are the recent e
Hydrogen sulfide(H2S)is an important endogenous signalling molecule with multibiological functions.Studies have shown that H2S level is correlated with nume
本研究采用生物信息学和分子生物学结合的方法,发现和鉴定人类基因组中肿瘤相关的反式作用小干扰RNA (tasiRNA)基因同系物.在完成人类基因组,MPSS和小RNA数据的采集和预处理
Aptamers,which can be generated via systematic evolution of ligands by exponential enrichment(SELEX),are superior ligands for molecular recognition due to t