Dual sgRNA-directed gene knockout using CRISPRCas9 technology in Caenorhabditis elegans

来源 :第五届中国小核酸技术与应用学术会议 | 被引量 : 0次 | 上传用户：lfq_1

【摘要】

：

　　The CRISPR RNA-guided Cas9 nuclease gene-targeting system has been successfully used for genome editing in a variety of organisms.Here,we report the use of

【作者】

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Xiangyang Chen Fei Xu Chengming Zhu Jiaojiao Ji Xufei Zhou Xuezhu Feng Shouhong Guang

【机构】

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SchoolofLifeSciences,UniversityofScienceandTechnologyofChina,Hefei,Anhui230027,P.R.China

【出处】

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第五届中国小核酸技术与应用学术会议

【发表日期】

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2015年9期

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　　The CRISPR RNA-guided Cas9 nuclease gene-targeting system has been successfully used for genome editing in a variety of organisms.Here,we report the use of dual sgRNA-guided Cas9 nuclease to generate knockout mutants of protein coding genes,noncoding genes,and repetitive sequences in C.elegans.

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Dual sgRNA-directed gene knockout using CRISPRCas9 technology in Caenorhabditis elegans

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