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Radio Frequency Identification (RFID) technology has been widely applied in many scenarios such as inventory control, supply chain management due to its superior properties including fast identification and relatively long interrogating range over barcode systems.It is critical to efficiently identify the unknown tags because these tags can appear when new tagged objects are moved in or wrongly placed.The state-of-the-art Basic Unknown tag Identification Protocol-with Collision-Fresh slot paring (BUIP-CF) protocol can first deactivate all the known tags and then collect all the unknown tags.However, BUIP-CF protocol investigates an ALOHA-like technique and causes too many tag responses, which results in low efficiency.This paper proposes a Fast Unknown tag Identification (FUI) protocol which investigates an indicator vector to label the unknown tags with a given accuracy and removes the time-consuming tag responses in the deactivation phase.FUI also adopts the classical Enhanced Dynamic Framed Slotted ALOHA (EDFSA) protocol to collect the labeled unknown tags.We then investigate the optimal parameter settings to maximize the performance of the proposed FUI protocol.Extensive simulation experiments are conducted to evaluate the performance of the proposed FUI protocol and the experimental results show that it considerably outperforms the state-of-the-art protocol.