QUANTITATIVE PROTEOMIC ANALYSIS FOR THE IDENTIFICATION OF CANDIDATE BIOMARKERS IN BUCCAL SQUAMOUS CE

• 作 者: NingLi;WenLiu;LijuanZeng;FeiWang;CanhuaJiang;FengGuo;
• 机 构: Xiangya Hospital,Changsha,Hunan,China
• 出 处: 第九次全国口腔颌面-头颈肿瘤学术研讨会
• 发表日期: 2015年期

　　Background:The objective of this study was to discover promising biomarkers for buccal squamous cell carcinoma(BSCC)patients arising in the background of oral submucous fibrosis(OSF)by using quantitative proteomics.Materials and Methods: BCSS,OSF,and normal buccal tissue(NBM)were collected for proteomics analysis.Proteins from each group were labelled with isobaric tags for relative and absolute quantitation(iTRAQ),then analyzed by LC-MS/MS.The evaluation of candidate biomarkers includes Western blotting and immunohistochemistry,then a clinical validation of promising biomarkers in a retrospectively study.