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Natural protoberberine (bis-benzyl-isoquinoline) alkaloids are widely used in the therapy of various diseases in folk medicine in West Africa.When extracted from the Enantia species,they have been used for liver diseases.In this study,we have taken a batch from the bark of Enantia chlorantha,Oliv (Annonaceae) separated with the method used for quercetin in pharmacognosy and then analyzed the mixture with GC-chromatography.The results obtained were as follows:63% palmatine,8% columbamine,19% jatrrorhizine,9% pseudocolumbamine,and 1% pseudopalmatine.The most abundant palmatine is also sensitive to hydride transfer and thus it regulates the natural equilibrium,when the alkaloid balance is artificially disturbed.The electron transfers to the alkaloid mixture were induced when the alkaloid mixture was evaporated from the polar solvent causing an energy transfer on a higher energy level and a transition seen as a broader bandwidth and a slightly retarded band in the UV-capillary electropherogram spectra.The bandwidth in the capillary zone electropherogram was caused by the several transfer electrons packed into the same transition level,which led to the congestion and diversity.This obtained transfer congestion is,however,very sensitive and describes how the evaporation of the polar solvent was strong enough to change the therapeutic properties even 100 times.The influence of the alkaloid mixture produced was tested in D-GalN liver-injured laboratory rats,which mimics the viral injury caused by sexually transmitted hepatitis B.