Cloning, expression and functional analysis of mamZ gene participating in magnetosome biosynthesis o

• 作 者: FengLi;
• 机 构: College of Life Sciences,Huaibei Normal University,Huaibei 235000,Anhui,P.R.of China
• 出 处: 第十七次全国环境微生物学学术研讨会
• 发表日期: 2014年期

　　For seeking genes associating with magnetosome biosynthesis mechanism in magnetotactic bacteria cells,a magnetosome-deleted NM7 of Magnetospirillum gryphiswaldense MSR-1 was obtained by mini-Tn5 lac Z2 transposon insertion mutation of conjugation experiment,and a 4642-bp fragment containing mini-Tn5 lac Z2 in NM7 was cloned includes the sequential steps of digestion,self-ligation,transformation and complete sequencing.