Altered expression of miR-21 is related to PTEN expression and clinicopathologic features of colorec

• 作 者: Wu yiying Zhu ling Xiong yao Liu litao Liu zhenru Sun yun Zhang yuanyuan Zhou liming
• 机 构: Department of Pharmacology,West China School of Preclinical and Forensic Medicine,Sichuan University
• 出 处: 2013医学前沿论坛暨第十三届全国肿瘤药理与化疗学术会议
• 发表日期: 2013年12期
• 关键词: colorectal carcinoma miR-21 PTEN

Objective To investigate the expressions of microRNA-21 (miR-21) and PTEN in colorectal carcinoma (CRC),and explore their correlation.Method Real-time PCR was used to detect the expression of miR-21 in 105 cases of colorectal carcinoma and tumor-adjacent normal tissue after operation.Moreover,16 pairs of colorectal carcinoma and normal tissues were chosen to evaluate the PTEN protein levels by western blot.Construct the recombinant eukaryotic expression and short hairpin (shRNA)expreesion vectors of microRNA-21 and PTEN,and transfect them into human colorectal cancer cells.Then,real-time PCR and western blot were used to detect the expression of PTEN mRNA and protein,respectively.