【摘 要】
：
Cyclodextrins (CDs) was cyclic oligosaccharides consisting of six or more α-(l,4)-linked D-glucopyranose units.The β-cyclodextrin molecule had secondary 2-and 3-hydroxyl groups lining the mouth of the
【机 构】
：
Beijing Key Lab of Bioprocess,College of Life Science and Technology,Beijing University of Chemical
【出 处】
：
The 6th International Conference on Separation and Technolog
论文部分内容阅读
Cyclodextrins (CDs) was cyclic oligosaccharides consisting of six or more α-(l,4)-linked D-glucopyranose units.The β-cyclodextrin molecule had secondary 2-and 3-hydroxyl groups lining the mouth of the cavity and primary 6-hydroxyl groups at the rear of the molecule.The cavity permitted inclusion of hydrophobic portions of the solute molecules.The monolithic column was prepared in a 100 mm × 4.6 mmi.d.stainless steel chromatographic column tube by an in situ polymerization technique.The porogenic solvent was a mixture of toluene and heptane,functional monomer and cross-linker were glycidyl methacrylate (GMA) and ethylene glycol dimethacrylate (EGDMA) respectively,and 2,2′-azobisisobutyronitrile (AIBN) was initiator.Scanning electron microscope (SEM) showed that the monolithic material was homogeneous with highly interconnected pores,forming a network of channels (Figure 1).The monolith skeleton in the dry state was characterized by mercury intrusion porosimeter,and showed a specific surface area of 5.11 m2 g-1,average pore size of 1.12 μm,and a total porosity of 65.6%.Oligo-β-cyclodextrin was coupled to the stationary phase for a rapid separation and determination of puerarin from Radix pueradae isoflavonoids.The polymerization conditions,including the ratio of functional monomer and cross-linker,the porogenic solvent,polymerization time and temperature,coupling concentration,temperature and time,were investigated to obtain an efficient monolith.The chromatographic performance of the monolith was evaluated in high performance liquid chromatography (HPLC).This study has shown that the oligo-β-cyclodextrin bonded monolithic column has a potential application in the rapid separation and determination of natural products.
其他文献
Enantiomer separation is one of the most important prerequisites for the investigation of environmental enantioselective behavior for chiral pesticides [1-2].In the present study,HPLC is one of the mo
The extraction of caffeine from Korean green tea was performed by various extraction methods such as dipping,stirring,ultrasonic and supercritical CO2 extraction.The extracts were analyzed by reversed
4-nitrochlorobenzene and 2-nitrochlorobenzene are important raw materials for the chemical industry.The present actual production condition,4 main production methods of them in China and abroad are zo
Hydrogen is increasingly demanded in various fields,including fuel cells,semiconductor processing,and the petrochemical industry.When steam reforming technology has been applied to produce hydrogen,it
As a new highly active adsorbent,the adsorption performance of lanthanum alginate bead has been reported.The adsorption mechanism was discussed in this study,including isotherm model fitting,XRD,FTIR
Chamaecyparis obtusa (Cupressaceae) is an evergreen conifer and a major plantation species for timber production in Japan,China,and Korea.The species has been reported to have antimicrobial and insect
A monolithic molecularly imprinted polymer (monofithic MIP) for dibenzoyl-D-tartaric acid (D-DBTA) was prepared by in situ polymerization as HPLC stationary phase.By optimizing polymeric and chromatog
A numerical model is developed to describe the counter current chromatography (CCC) separation process in this work.The theory of counter current extraction [1] is firstly introduced to calculate conc
Carbon black has been widely used in composites,toner,resin,and ink materials.Carbon black can be characterized by particle size structure and surface functional group.Physical properties of the carbo
Titanium dioxide (TiO2) powders were synthesized by using TiO2 colloidal sol prepared from titanium isopropoxide (TTIP) used as a starting material by applying the sol-gel methodL The effect of aging