【摘 要】
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A micro-/nano-fluidic device was designed and used to quantify highly reactive oxygen species(hROS)in the mainstream of cigarette smoke(CS)based on surface plasmon resonance-enhanced fluorescence(SPRE
【机 构】
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State Key Laboratory of Analytical Chemistry for Life Science,School of Chemistry and Chemical Engin
【出 处】
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第九届全国微全分析系统学术会议、第四届全国微纳尺度生物分离分析学术会议、2014国际微流控芯片与微纳尺度生物分离分析学术
论文部分内容阅读
A micro-/nano-fluidic device was designed and used to quantify highly reactive oxygen species(hROS)in the mainstream of cigarette smoke(CS)based on surface plasmon resonance-enhanced fluorescence(SPREF)effect.The micro-/nano-fluidic device integrated with nanochannels,chip electrophoresis and a LIF detector.Using this device,core-shell Ag@SiO2 nanoparticles can be effectively concentrated in the front of the nanochannel via size exclusion effect.The concentrated Ag@SiO2 nanoparticles will effectively enhance the fluorescent intensity of fluorophores nearby.Thus,sensitively detection of oxidative activity of CS has been achieved based on the cleavage of DNA oligomers labeled with fluorophores(FAM)(Fig.1).
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