Modified disposable pipette extraction(DPX)coupled with liquid chromatography-electrospray-mass spec

来源 :第34届(2016)中国质谱学会学术年会 | 被引量 : 0次 | 上传用户:O70607227
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  A simple method for simultaneous determination of 14 perfluorinated compounds(PFCs)in the vegetable samples was developed and validated.The method adopted ultrasonic-assisted extraction followed by modified disposable pipette extraction(DPX)coupled with ultra performance liquid chromatography-tandem mass spectrometery using a fused-core analytical column for determination of 14 PFCs.The quantification was performed by using matrix-match calibration curve with internal standards.The two ultrasonic-assisted extractions were executed.The high extraction efficiency was obtained when the amount of 100 mg graphitized carbon was added to clean-up.The linearity of the calibration curve was greater than 0.99 for all analytes in five days.The limit of detection(LOD)and limit of quantification(LOQ)ranged from 0.006 ng/g to 0.14 ng/g and from 0.059 ng/g to 0.47 ng/g,respectively.The extraction recoveries were in the range of 83.83%to 112.00%and the extraction recoveries didnt depend on spiked levels.The matrix effects cloud be negligible for all analytes.The within-run precision and between-run precision were <20%for each analyte.The within-run accuracy and between-run accuracy were between-10.52%and+13.00%.The method was applied for determination of PFCs in vegetables.The five analytes were detected in some vegetables,but at low levels.The average values of relative recovery were obtained from 80%to 120%in vegetables.Good results on linearity,LOQ,extraction recovery,matrix-effects,precision and accuracy were achieved,indicating that the method can be used for simultaneous determination of 14 PFCs in vegetables.
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