柑橘黄龙病症状类型与PCR检测结果的相关性分析

来源 :中国植物病理学会2015年学术年会 | 被引量 : 0次 | 上传用户:gyquan
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  柑橘黄龙病是威胁世界柑橘生产的最严重的病害.黄龙病在柑橘上的症状较为复杂,因寄主品种、季节、叶片生长期、病程等因素而异.本研究首先证明直接从症状上判断一样品是否感染黄龙病是不可靠的(假阳性率为8.20%,假阴性率达50%),需要进一步研究症状与病害的相关性,以提高田间黄龙病诊断的准确率.其次,收集1500个样品信息,分析症状与病原检测结果的相关性.提出"斑驳"、"均匀黄化"和"绿岛"这3种叶片症状和"红鼻子果"和"畸形果"这2种橘类果实症状是与黄龙病相关性最高的症状,是田间黄龙病的快速诊断的最可靠依据.斑驳症状的强弱与病原的定性检测结果相关.苗木、接穗和果实的病害检测应优先选用实时荧光定量PCR.本研究证实砧木本身对黄龙病的抗耐性差异显著,耐病砧木品种中的相关耐病基因资源的挖掘与利用是一个新的研究方向.
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