Analysis of screening and bioinformatics of antithrombotic peptides in casein molecules from bovine

• 作 者: MaolinTu[1]Liting.Feng[1]Zhenyu.Wang[2]Meiling.Qiao[2]Weihong.Lu[1]Ming.Du[1]
• 机 构: Department of Food Sciencc and Engineering,Harbin Institute of Technology Harbin 150090,China
• 出 处: 第八届国际分子模拟与信息技术应用学术会议
• 发表日期: 2016年期

　　Thrombotic disease.caused by thrombosis and thromboembolism,is seriously harmful to human health and is one of the major causes of death in modern society.Blood clotting can be prevented by anticoagulant drugs by influencing some impact factors in the blood coagulation process.Food-derived biological peptides with anticoagulant activity have many advantages.such as fast absorption and low side effects.which are attracting more and more attentions on anticoagulants studies.Casein is a good source of bioactive peptides.In the present study,αs1-,αs2-,β-and κ-casein were selected for in silico digestion,and the theoretic peptides from them were predicted.Antithrombotic peptides from αs1-,αs2-,β-and κ-casein were screened by bioinformatics analysis based on the amino acid sequences of antithrombotic peptides reported.Molecular docking.using Accelrys Discovery Studio software.was adopted to confirm some new anticoagulant peptides.One peptide from αs2-casein,Ser-Ser-Glu-Glu-Ser-Ile-Ile-Ser-Gln-Glu-Thr-Tyr-Lys-Gln-Glu-Lys-Asn-Met-Asp.showed better affinity activity with thrombin based on the results of bioinformatics analysis.The result from this study could provide some theoretical information for the application of antithrombotic peptides in functional food industry especially in dairy nutrition.