【摘 要】
：
The primary objective of the current study is to define the role of miRNA-320 in determining sensitivity of cervical cancer to radiotherapy.To explore the mechanism and to evaluate the potential of Mi
【机 构】
：
Department of Radiation and Medical Oncology, Zhongnan Hospital, Wuhan University, 169 Donghu Road,W
【出 处】
：
第九届泛珠江区域放射肿瘤学学术大会暨肿瘤放射治疗多中心协作研讨会、重庆市医学会放射肿瘤治疗学专业委员会2014年会
论文部分内容阅读
The primary objective of the current study is to define the role of miRNA-320 in determining sensitivity of cervical cancer to radiotherapy.To explore the mechanism and to evaluate the potential of Mi(e)roRNA-320(miRNA-320) as a biomarker for predicting the radiosensitivity, we established a radioresistant cervical cancer cell line, C33AR.The real-time qPCR results revealed that miRNA-320 was differentially expressed between C33AR and its parental cell lines C33A.We found that the expression of miRNA-320 was obviously decreased in C33AR.Furthermore, we explored colony-formation assay and the result indicated that C33AR was more radioresistant.A target prediction suggests that miRNA-320 negatively regulate the expression of β-catenin, a mediator of adherence junctions with α-catenin, and a central player of the canonical Wnt signaling pathway, which is related to the survival of stem cells and also correlated with malignant tumor behaviours.On the other hand, overexpressing miRNA-320 could increase C33AR radiosensitivity.Our data showed that miRNA-320 was a potential biomarker for radiosensitivity.
其他文献
Objective: The purpose of this study was to evaluate the early changes of ADC value after radiation and correlation between ADC value and MMP-9, PAI-1 expression level in the irradiation mouse model.M
Cancer patients often suffer from local tumor recurrence after radiation therapy.Cell cycle, an intricate sequence of events which guarantee the high genomic fidelity, has been suggested to affect DNA
目的：探讨TLR4基因RNA干扰(RNAi)的重组慢病毒载体对人乙肝病毒相关肝细胞癌裸鼠移植瘤TLR4基因表达和移植瘤生长的影响.方法：构建4个miR-TLR4质粒和一个阴性对照质粒,选择干扰效果最明显的质粒进行慢病毒包装.建立人乙肝病毒相关肝细胞癌裸鼠移植瘤模型,随机分成实验组、阴性对照组和空白对照组.隔天1次向各组动物肿瘤内分别注射TLR4 miRNA慢病毒颗粒、携带无关序列的慢病毒颗粒或0.
背景与目的：目前鼻咽癌公认和有效的根治性手段为放射治疗或以放疗为主的综合治疗.放射线抗拒是鼻咽癌治疗失败的主要原因,研究其抗拒机制、寻求一些辅助手段或药物降低肿瘤细胞的放射抵抗、提高放射敏感性、提高疗效.CCDC7蛋白家族均含有一个CCDC7保守结构域,在进化上高度保守,在高等哺乳动物中高度同源,但其功能知之甚少.目前关于该基因与鼻咽癌的关系国内外尚未见报道.目的：研究CCDC7基因增强鼻咽癌细胞
目的：检测端粒结合蛋白CTC1的表达与人类恶性黑色素瘤细胞放射敏感性之间的关系并探讨其潜在的作用机制.方法：以人恶性黑色素细胞株MDA-MB-435为研究对象,采用X射线分次外照射模式,构建人放射敏感/放射抗拒黑色素瘤细胞模型——MDA-MB-435/MDA-MB-435R.克隆形成实验比较两种细胞的放射敏感性差异.Realtime PCR和western blot分别检测两种细胞中CTC1的mR
肿瘤细胞对TRAIL的耐受限制了其广泛应用于临床.TRAIL耐受的相关机制大部分集中在TRAIL的原发性耐受方面,有关肿瘤细胞获得性TRAIL耐受的发生发展机制方面的研究非常少见.在我们的研究中,为了研究获得性TRAIL耐受的分子机制,我们以TRAIL敏感的非小细胞肺癌H460细胞为基础,通过无限稀释克隆形成实验和低浓度TRAIL诱导,建立并成功鉴定获得性TRAIL耐受细胞株模型H460R.同时我
目的：探讨瘦素及瘦素受体与食管鳞状细胞癌之间的关系,为食管癌的防治提供科学的依据.方法：收集2009年12月到2010年11月间山西省肿瘤医院胸外二科32例食管鳞状细胞癌病人,以20例健康人为对照,采用免疫组织化学染色和ELSIA测定食管组织中瘦素及其受体的表达情况,分析其与临床病理因素的关系.结果：食管鳞癌组织及正常食管组织中瘦素水平分别为(4.7±1.9)ng/ml和(2.54±1.01)ng
目的：研究显示Bmi-1高表达与食管癌放射抵抗密切相关,生物信息软件分析示Bmi-1是miR-203的靶基因,本研究探索miR-203是否可通过下调Bmi-1的表达来增加食管鳞癌TE-1细胞的放射敏感性.方法：应用miRNA芯片分析了6例临床上放射敏感与不敏感的食管鳞癌病人活检组织标本差异miRNA的表达情况,结果显示miR-203在放射敏感组中高表达且倍数大于2.5倍.用Lipofectamin
Autophagy has been recently suspected to plays an important role in the regulation of cancer development and the response of tumor cells to anticancer therapy.However, recent studies showed that autop
背景 斯钙素2(Stanniocalcin 2,STC2)在多种恶性肿瘤中过度表达,其过度表达大多与肿瘤的进展及不良预后有关.然而,在鼻咽癌中STC2的表达情况及临床意义仍未见报道.本研究检测了94例鼻咽癌组织中STC2的表达情况,并进一步分析了STC2表达的临床诊断价值及其对预后的影响.方法 本研究回顾性选取2008年1月至12月于我院初诊的鼻咽癌患者,均经过病理证实为低分化或未分化癌,临床各项