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目的:建立复方雄蚕蛾胶囊质量控制的定性定量方法。方法:总多糖含量测定采用苯酚-硫酸显色法[1],在490nm波长处测定吸收度,在0.02~0.1g.L-1范围内,其质量浓度与吸光度线性关系良好(r=0.9984);蛋白质含量测定采用凯氏定氮法[2]。结果:复方雄蚕蛾胶囊中总多糖的平均含量为5.9%,蛋白质平均含量为51.3%。结论:该方法操作简便、快速、灵敏度高和重复性好,可以作为复方雄蚕蛾胶囊的质量控制的定性定量方法。
Objective: To establish a qualitative and quantitative method for quality control of compound male silkworm moth. Methods: The total polysaccharide content was determined by phenol-sulfuric acid spectrophotometry [1], and the absorbance was measured at 490 nm. The linear range was 0.02-0.1 gL-1 and the linearity was good (r = 0.9984) Determination of the content of Kjeldahl method [2]. Results: The average content of total polysaccharides in compound male silkworm moth capsule was 5.9% and the average content of protein was 51.3%. Conclusion: The method is simple, rapid, sensitive and reproducible. It can be used as a qualitative and quantitative method for the quality control of compound male silkworm moth.