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Dissolving microneedles as a transdermal drug preparation;this can penetrate the corneum and effectively improve the transdermal rate of drugs.However, the penetrating site had not been investigated yet.The goal of this study is to investigate whether the specific site of acupoints can play an important role in immune cell proliferation as acupuncture done.To examine the immune cells proliferation, the SD rats were made into immunosuppression model by cyclophosphamide and divided into 3 groups.Among them, one group gave acupuncture, one group gave microneedle, and both two groups were applied on ZuSanLi acupoints and continuous administration for 10 days.The rest of the group without any treatment.After the experiment, extracting the spleen cells and assaying the cells proliferation by CCK8.The results from enzyme-labelled show that the OD value of acupuncture group, microneedle group and blank group reached 0.68±0.01, 0.456±0.03,0.247±0.02.The ratio of spleen cell proliferation of acupuncture group and microneedle group present 435.66%, 263.57%.The spleen cell proliferation rate of microneedle group is much less than acupuncture group, but both of two groups can cause immune cell proliferation.It demonstrate that microneedle without encapsulated drug can also cause immune cell proliferation as traditional acupuncture when applicate on special site.In conclusion, the dissolving microneedle array applicate on special site could be great potential for future clinical application.