A TWO-STAGE TEMPERATURE CONTROL STRATEGY FOR EFFICIENT SECRETION OFRECOMBINANT CYCLODEXTRIN GLYCOSYL

• 作 者: Jing Wu Zhaofeng Li Bin Li Cheng Cheng Jian Chen
• 机 构: State Key Laboratory of Food Science and Technology ; School of Biotechnology, Jiangnan University,
• 出 处: 第十四届世界食品科技大会
• 发表日期: 2008年1期

　　For the production of industrial enzymes through genetic engineering,E.coii remains an attractive expression system owing to its simplicity.Since most industrial enzymes are extracellular proteins,considerable efforts have been made to target recombinant proteins outside the cells,mostly to the periplasmic space.