【摘 要】
：
目的:观察米非司酮(mifepristone,MIF)对慢性应激大鼠脂代谢和肝脏中GRmRNA、PPAR-γmRNA表达的影响.方法:采用高脂饲料联合四种不同的应激方式(悬尾30 min;PVC筒固定2h;纱布束缚2h;大鼠固定架制动2h)建立慢性应激大鼠模型.随机分为正常对照(control)组、应激模型(model)组、米非司酮-低剂量(MIF-L)(10 mg·kg-1·d-1)、中剂量(M
【机 构】
：
兰州军区兰州总医院国家中医药管理局临床中药学重点学科,甘肃兰州730050;兰州大学药学院,甘肃兰州730000
【出 处】
：
世界中医药学会联合会中药药理专业委员会第七届学术会议、全国中药药理联合会第二届学术年会暨第20届中日健康学术研讨会
论文部分内容阅读
目的:观察米非司酮(mifepristone,MIF)对慢性应激大鼠脂代谢和肝脏中GRmRNA、PPAR-γmRNA表达的影响.
方法:采用高脂饲料联合四种不同的应激方式(悬尾30 min;PVC筒固定2h;纱布束缚2h;大鼠固定架制动2h)建立慢性应激大鼠模型.随机分为正常对照(control)组、应激模型(model)组、米非司酮-低剂量(MIF-L)(10 mg·kg-1·d-1)、中剂量(MIF-M)(25mg·kg-1·d-1)、高剂量(MIF-H)(50 mg·kg-1·d-1)组,正常组和模型组给予等量蒸馏水.每周测定每组大鼠平均饮水量、摄食量、大鼠体重变化,给药4周后断头处死,测定血清中血脂四项(HDL-C、LDL-C、TG、TC)水平,HPA轴激素(CRH、ACTH、CORT)水平,采用Real Time PCR测定大鼠肝脏组织中GRmRNA,PPAR-ymRNA的表达.
结果:相比control组,model组TG、TC、LDL-C、CRH、ACTH、CORT升高;与model组比,MIF-L降低TC,升高HDL-C,MIF-M、MIF-H两组对TC、TG均有改善作用,且差异有显著性(P＜0.04,P＜0.05),MIF-M降低LDL-C;MIF降低CRH、ACTH水平,升高CORT水平,且MIF-H组CORT升高差异有显著性(P＜0.01).
结论:MIF改善应激大鼠脂代谢紊乱,其有可能是通过升高肝脏中GRmRNA和PPAR-ymRNA的表达发挥作用.
其他文献
Aim of study:In the past,overeating was regarded as the main cause of increasing adiposity,but now a new viewpoint from JAMA suggested that energy metabolic defects may precede overeating in obesity.M
Objective:In order to evaluate protective Effect of different compatibility proportion of Aconiti Lateralis Radix Praeparata and Ginseng Radix et Rhizoma hypoxia injury in neonatal rats cardiomyocytes
Aim of study:To observe the liver toxicity of matrine and to explore the related mechanisms,so as to provide the experimental basis for its clinical safety medication.Material and methods:L-02 hepatoc
Objective:To conduct study on the protective effect of Tibetan Rannasangpei Pill administered on a one-time treatment of cerebral ischemia /reperfusion injury and to explore the time-effect relationsh
Coronary artery heart disease is a cardiac dysfunction and organic disease,which main pathological states are coronary artery stenosis,angina,myocardial infarction,myocardial ischemia,heart failure an
Aim of the study:To evaluate the effects of TQHXD on the Blood-Brain Barrier (BBB) permeability and the expressions of related proteins on the rats and analyse the constituents in the cerebrospinal fl
Aim:Compound Radix Salviae Miltiorrhizae Tablets,also known as Fufang Danshen Pian (DSP) in China,has been commonly used in traditional Chinese medicine to prevent and treat cardiovascular diseases (C
Aim of study:Parkinsons disease (PD) is the second most common chronic neurodegenerative disorder,after Alzheimers disease.Its pathological feature is damage to the nigrostriatal dopaminergic pathway.
目的:研究参附汤血中移行成分对血管紧张素Ⅱ(AngⅡ)所致心肌细胞凋亡及自噬的影响.方法:采用原代培养乳鼠心肌细胞,随机分为空白组、AngⅡ组、移行成分组.运用MTT比色实试验法测定心肌细胞存活率,采用实时定量PCR检测凋亡相关基因Caspase-3和自噬相关基因Beclin1、Atg5mRNA水平在不同组中的表达.结果:与正常组比较,血中移行成分组细胞存活率无明显变化(P＞0.05),与模型组比
目的:探讨长期血糖平均水平不同的2型糖尿病(Type 2 diabetes mellitus,T2DM)患者下丘脑-垂体-肾上腺(Hypothalamic-pituitary-adrenal,HPA)轴激素与褪黑素(Melatonin,MLT)的关系,了解褪黑素在糖尿病发病进程中的变化.方法:将90例2型糖尿病患者根据糖化血红蛋白(HbA1c)水平分为三组(A组:HbA1c＜7％,B组:7％≤Hb