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Aim of study:To observe the liver toxicity of matrine and to explore the related mechanisms,so as to provide the experimental basis for its clinical safety medication.Material and methods:L-02 hepatocytes,with good growth state,were digested by trypsin,centrifugalized,resuspended and adjusted the cell density to 1 x 106/ml.The cells were cultured in a 6 well plate,with 5%CO2 at 37℃.The cell growth curve was analyzed by MTT method.After the cells adhered and in a logarithmic growth phase,the test drug was intervened.4 groups were designed,namely negative control group,matrine 3.2mmol/L group,8.1mmol/L group and 20.2mmol/L group,each group with 5 holes.The cell growth was observed 24h after drug intervention with inverted microscope.IC50 was calculated with MTT method.The culture liquid was collected for measuring ALT,AST,ALP,LDH value with automatic biochemical analyzer.The cell climbing piece technology was applied,the cells were stained with HE,and the morphological changes were observed under light microscopy.The cell apoptosis was observed by DAPI staining method.The cell contents of MDA and GSH were determined by the method of Elisa.Results:Within the range from 3.2 to 20.2 mmol/L concentrations,the proliferation of L-02 hepatic cells was inhibited by matrine in a concentration dependent manner,with the IC50 0f 5.3mmol/L.Compared with that in the negative control group,the activities of ALT,AST,ALP and LDH of the cultural medium were increased in the matrine treated groups,and with a concentration dependent manner in the range of 3.2-20.2mlM/L.The morphology of the L-02 liver cells was changed in the high concentration of matrine,with cell swelling,and nucleus disappeared.Compared with that in the negative control group,the MDA content in the high concentration of matrine group was increased (P<0.05),and the content of GSH was decreased (P<0.05).DAPI staining results showed that high concentration of matrine could partially cause L-02 liver cell rupture and apoptosis.Conclusions:The high concentration of matrine has toxic effect on L-02 liver cells,resulting in cell proliferation inhibition,ALT,AST,ALP,LDH spillover and cell morphological changes.The preliminary research shows that the mechanisms of the toxicity of matrine on liver cells may be associated with peroxidation and cell apoptosis.