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To determine if Chinese and Japanese wheat landraces and varieties have unique sources of Fusarium head blight (FHB) resistance, an association mapping panel of 195 wheat accessions including both commercial varieties and landraces was genotyped with 364 genome-wide simple sequence repeat (SSR) and sequence-tagged site (STS) markers, and evaluated for type Ⅱ FHB resistance in three greenhouse experiments using single floret inoculation.